multi drug resistant human uterine sarcoma cells mes sa dx5 Search Results


multi drug resistant human uterine sarcoma cells mes sa dx5  (ATCC)
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ATCC multi drug resistant human uterine sarcoma cells mes sa dx5
Multi Drug Resistant Human Uterine Sarcoma Cells Mes Sa Dx5, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell counting kit-8  (Dojindo Labs)
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Dojindo Labs cell counting kit-8
Cell Counting Kit 8, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3e, mouse(145-2c11)  (Biotium)
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Biotium cd3e, mouse(145-2c11)
Cd3e, Mouse(145 2c11), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-h-2d b  (Becton Dickinson)
90
Becton Dickinson anti-h-2d b
Anti H 2d B, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ps6  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc ps6
NK cell phenotype and <t>pS6</t> expression upon retrovirus infection. Splenocytes of naïve and FV-infected animals (7 dpi) were stained for NK cell markers (NK1.1 + , CD49b + , CD3 - ) and analyzed for activation (CD69, A ) and cytotoxicity (GzmB, B, C ). At least nine mice per group from three independent experiments were used. Statistically significant differences were analyzed by Mann-Whitney test (A) or unpaired t test (B) . NK cells were stained for pS6 in naïve and mice from 7 dpi, which expression is shown as a representative histogram in (D) A kinetic of NK cells expressing pS6 is shown in (E) NK cells were analyzed at 0 (22 mice), 7 (17 mice), 14 (9 mice) and 28 dpi (6 mice). NK cells from 0 and 7 dpi were compared and statistically significant differences were analyzed with an unpaired t test. Data are presented as mean values ± SEM.
Ps6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ps6/product/Cell Signaling Technology Inc
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anti mouse major histocompatibility complex mhc class i h2ld  (OriGene)
99
OriGene anti mouse major histocompatibility complex mhc class i h2ld
NK cell phenotype and <t>pS6</t> expression upon retrovirus infection. Splenocytes of naïve and FV-infected animals (7 dpi) were stained for NK cell markers (NK1.1 + , CD49b + , CD3 - ) and analyzed for activation (CD69, A ) and cytotoxicity (GzmB, B, C ). At least nine mice per group from three independent experiments were used. Statistically significant differences were analyzed by Mann-Whitney test (A) or unpaired t test (B) . NK cells were stained for pS6 in naïve and mice from 7 dpi, which expression is shown as a representative histogram in (D) A kinetic of NK cells expressing pS6 is shown in (E) NK cells were analyzed at 0 (22 mice), 7 (17 mice), 14 (9 mice) and 28 dpi (6 mice). NK cells from 0 and 7 dpi were compared and statistically significant differences were analyzed with an unpaired t test. Data are presented as mean values ± SEM.
Anti Mouse Major Histocompatibility Complex Mhc Class I H2ld, supplied by OriGene, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse qa-1[b]-biotin  (Becton Dickinson)
90
Becton Dickinson anti-mouse qa-1[b]-biotin
NK cell phenotype and <t>pS6</t> expression upon retrovirus infection. Splenocytes of naïve and FV-infected animals (7 dpi) were stained for NK cell markers (NK1.1 + , CD49b + , CD3 - ) and analyzed for activation (CD69, A ) and cytotoxicity (GzmB, B, C ). At least nine mice per group from three independent experiments were used. Statistically significant differences were analyzed by Mann-Whitney test (A) or unpaired t test (B) . NK cells were stained for pS6 in naïve and mice from 7 dpi, which expression is shown as a representative histogram in (D) A kinetic of NK cells expressing pS6 is shown in (E) NK cells were analyzed at 0 (22 mice), 7 (17 mice), 14 (9 mice) and 28 dpi (6 mice). NK cells from 0 and 7 dpi were compared and statistically significant differences were analyzed with an unpaired t test. Data are presented as mean values ± SEM.
Anti Mouse Qa 1[B] Biotin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NK cell phenotype and pS6 expression upon retrovirus infection. Splenocytes of naïve and FV-infected animals (7 dpi) were stained for NK cell markers (NK1.1 + , CD49b + , CD3 - ) and analyzed for activation (CD69, A ) and cytotoxicity (GzmB, B, C ). At least nine mice per group from three independent experiments were used. Statistically significant differences were analyzed by Mann-Whitney test (A) or unpaired t test (B) . NK cells were stained for pS6 in naïve and mice from 7 dpi, which expression is shown as a representative histogram in (D) A kinetic of NK cells expressing pS6 is shown in (E) NK cells were analyzed at 0 (22 mice), 7 (17 mice), 14 (9 mice) and 28 dpi (6 mice). NK cells from 0 and 7 dpi were compared and statistically significant differences were analyzed with an unpaired t test. Data are presented as mean values ± SEM.

Journal: Frontiers in Immunology

Article Title: Fatty acids are crucial to fuel NK cells upon acute retrovirus infection

doi: 10.3389/fimmu.2023.1296355

Figure Lengend Snippet: NK cell phenotype and pS6 expression upon retrovirus infection. Splenocytes of naïve and FV-infected animals (7 dpi) were stained for NK cell markers (NK1.1 + , CD49b + , CD3 - ) and analyzed for activation (CD69, A ) and cytotoxicity (GzmB, B, C ). At least nine mice per group from three independent experiments were used. Statistically significant differences were analyzed by Mann-Whitney test (A) or unpaired t test (B) . NK cells were stained for pS6 in naïve and mice from 7 dpi, which expression is shown as a representative histogram in (D) A kinetic of NK cells expressing pS6 is shown in (E) NK cells were analyzed at 0 (22 mice), 7 (17 mice), 14 (9 mice) and 28 dpi (6 mice). NK cells from 0 and 7 dpi were compared and statistically significant differences were analyzed with an unpaired t test. Data are presented as mean values ± SEM.

Article Snippet: For cytokine stainings, splenocytes were restimulated with ionomycin (500 ng/ml), phorbol myristate acetate (PMA; 25 ng/ml), monensin (1X, BioLegend), and brefeldin A (2 μg/ml) diluted in RPMI buffer at 37°C for 3 h. Multi-parameter flow cytometry was done with the following antibodies: CD3 (clone 145-2C11, Thermo Fisher), CD36 (HM36, BioLegend), CD49b (DX5, Thermo Fisher), CD69 (H1.2F3, BioLegend), GzmB (GB11, Becton Dickinson), IFN-γ (XMG1.2, Thermo Fisher), NK1.1 (PK136, Becton Dickinson), pS6 (D57.2.2E, Cell Signaling).

Techniques: Expressing, Infection, Staining, Activation Assay, MANN-WHITNEY